Abstract
Small non-coding RNAs, as class of small regulatory molecules, control normal development and differentiation of cells. Micro-RNAs and piwi-interacting-RNAs are members of epigenetic regulators family. In previous studies researches investigated the role of different small non-coding RNAs in pathophysiology of cancer. Objective: In this study A-549 lung adenocarcinoma cells were firstly epigenetically reprogrammed and transformed into CD4+ cells. Method: I used new non-viral carrier the complex of DDMC vector with antago-miR-155 and piR-30074 for long-lasting transfection of lung cancer cells. Results: The transformation of A-549 lung cancer cells was proved by morphological and genetic changes (AltAnalyze Platform) in dynamics. I observed CD4+ lymphocytes phenotypic marker and OCT4 marker of pluripotent cells by immunofluorescence microscopy in transformed cells.
Highlights
Lung cancer, which is leading cause of cancer death, is increasing in incidence
The DDMC vector was toxic to cells at a concentration more than 10 μg/ml (Figures 1A and 1B)
The toxicity was decreased after treatment with a complex of the DDMC vector and oligonucleotide (F observed ≤ F crit)
Summary
Lung cancer, which is leading cause of cancer death, is increasing in incidence. The SEER Stat Fact Sheets data statistics for lung cancer provide the following numbers: 221,200 estimated new cases in 2015 (% of all new cancer cases 13.3%); 158,040 estimated deaths in 2015 (% of all cancer deaths 26.8%); and 17.4% of patients surviving 5 years (2005-2011) [1]. The current lung cancer therapy has severe side effects and is often ineffective against tumors as the result of late diagnostics and/or metastases in the lymphatic system and in other organs [3,4,5,6]. For these reasons, it is necessary to investigate new anti-lung-cancer drugs. Small non-coding regulatory RNAs may be a new effective tool for lung cancer gene therapy. Complex of DDMC vector with two sncRNAs as candidate for future complex anti-lung cancer therapy was proposed and investigated in in vitro experiments
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