JIP analysis on rice (Oryza sativa cv Nipponbare) grown under limited nitrogen conditions

Abstract

Nitrogen deficiency significantly reduces the CO2 assimilation capacity of plants and the quantum yield of photosynthesis. Here, we employed the JIP test to determine the effects of nitrogendeficiency on the plant’s photosysnthetic ability on the basis of chlorophyll fluorescence. Nitrogendeficient and nitrogen-replete rice plants were analyzed for the fluorescence transients of the plant leaves in comparison with the nitrogen-sufficient controls. Results showed that 7 day-replete plants behaved normally while 5, 3, and 1 day-replete plants were significantly affected from nitrogen starvation. More specifically, nitrogen starvation of plants resulted in an inactivation of photosystem II (PS II) reaction centers and a decline in electron transport beyond the reduced plastoquinone (QAA −), and a decrease in both the pool size and the reduction of end electron acceptors at the PS I. The affected plants were fully recovered from the deficiency after 7 days of nitrogen repletion, as evidenced by the similar level of fluorescence transients to the positive controls. Thus, our results demonstrated that the movement of electron carriers leading to the reduction of end electron acceptors was affected by nitrogen limitation leading to a more pronounced decrease in the reduction of end electron acceptors. Together with the fact that nitrogen-deficiency limits the CO2 assimilation of plants, this study indicates that nitrogen metabolism is tightly coupled with photosynthetic ability.