Abstract
Objective To explore the effects and mechanism of Jinhong Tablet on intestinal mucosal barrier function and SIRS in rats with acute biliary infection. Methods 36 SD male rats were divided into three groups: sham operation (control), acute biliary infection (ABI) model, and Jinhong Tablet (Jinhong) group. Jinhong group were force-fed with Jinhong Tablet, while the other two groups received oral saline. At days 3 and 5, morphological changes of intestinal mucosa were assessed. Serum diamine oxidase (DAO), D-lactate, and endotoxin levels were measured. And the genes bcl-2 and bax in intestinal tissues were tested by real-time PCR and Western blotting. Results Intestinal damage was significantly less severe in Jinhong group compared with ABI group, as indicated by Chiu's scoring, TUNEL analysis, and serum DAO, D-lactic acid, and endotoxin levels. Additionally, the expression of bax mRNA and protein was decreased and the ratio of bcl-2/bax mRNA and protein was increased compared with ABI group. Conclusion Jinhong Tablet had a positive intervention on acute biliary infection through improving inflammation and intestinal mucosal barrier, inhibiting excessive apoptosis of intestinal epithelial cells via bax and bcl-2 gene, and protein regulation.
Highlights
Acute biliary infections are common diseases in surgery
The results show that serum diamine oxidase (DAO), D-lactic acid, and endotoxin levels in acute biliary infection group were significantly increased compared with control group at both days 3 and 5 (P < 0.01)
Clinical studies revealed that bacterial infections, necrosis, MODS, and multiple organ failure (MOF) induced by acute pancreatitis are closely related to intestinal mucosal barrier dysfunction in early disease stage
Summary
Acute biliary infections (cholecystitis, cholangitis, and biliary pancreatitis) are common diseases in surgery. Severe infections often lead to Systemic Inflammatory Response Syndrome (SIRS), which in turn causes multiple organ failure (MOF) with a high mortality rate [1, 2]. There are a variety of treatment methods, including anti-infection, antishock, surgery, and other comprehensive treatment schemes in view of local biliary diseases, but the effect is still unsatisfactory. Severe infection involved in other organs always leads to more serious body damage and the incidence of poor prognosis. The importance of intestinal mucosal barrier function in acute biliary infection has been recognized [3, 4]. Intestinal mucosal barrier can prevent intestinal bacteria and toxins from intestinal mucosa from entering into other organs and blood circulation in the body
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