Jingfang Granules May Improve Early Pancreatic Cancer by Modulating Immune Response Through RUNX3

Abstract

Objective: Jingfang granule (JFG) is a modified formulation of Jingfang Baidu Power for the treatment of influenza and infectious diseases. Recent studies show that JFG may inhibit the development of cancer but the effect of JFG on methylation in early pancreatic cancer has remained unclear. In this study, we aim to investigate the potential effect of JFG on gene methylation of early pancreatic cancer. Methods: We treated golden hamsters with N-Nitrosobis(2-oxopropyl)amine (BOP) to induce early pancreatic cancer and detected the gene methylation of pancreatic cancer tissue. The gene ontology (GO) analysis and the Kyoto Encyclopedia of Genes and Genome (KEGG) analysis were performed in this study. Results: The GO analysis demonstrated that there was no significantly correlated GO term enrichment from differentially methylated genes. And the KEGG analysis found that the top enriched term was the Th1 and Th2 cell differentiation pathway. Four differentially methylated genes were discovered in the Th1 and Th2 cell differentiation pathway of which RUNX3 was associated with pancreatic cancer development. Conclusion: The results investigate that JFG may improve early pancreatic cancer by activating the immune response by inhibiting RUNX3 methylation. This research provides a theoretical basis for the prevention and improvement of early pancreatic cancer in clinical treatment with traditional Chinese medicine.