Jianghuangsu exerts anti-proliferative and pro-apoptotic effects on HepG2 cells through extrinsic and intrinsic pathways

Abstract

Objective Detect the growth inhibition and apoptosis induction mechanisms of jianghuangsu on HepG2 cells and L-02 cells.Methods The rate of growth inhibition ( ％ ) of cultured HepG2 cells and L-02 cells was expressed by methyl thiazol tetrazolium ( MTT),cell cycle analysis and apoptosis rate were detected by fluorescence-activated cell sorting ( FACS),the expression of proteins were detected by immunocytochemistry staining or Western blotting.Result The flow cytometric analysis of HepG2 cells demonstrated that jianghuangsu primarily arrested the HepG2 cells at the G1 phase of the cell cycle.Annexin V-FITC/proliferation index (PI) staining corroborates the apoptogenic nature of jianghuangsu on HepG2 and L-02 cells was 25.89％ and 10.10％ at 100 mg/L,respectely.The growth inhibition ratio of HepG2 cells was 84％.The anti-proliferative and pro-apoptotic effect of jianghuangsuin HepG2 cells was associated with the activation of the mitochondria-mediated and death receptor-mediated apoptotic pathways.Conclusion Jianghuangsu has selective cytotoxic activities against HepG2 cell,it triggered time and dose dependent apoptosis on HepG2 cells by activating the mitochondria-mediated and death receptor-mediated apoptotic pathways. Key words: Jianghuangsu ; HepG2 cells; Apoptosis ; Caspase