Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil

Abstract

A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2(T), was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2(T) was able to grow in the presence of 0-23 % (w/v) NaCl and at pH 4.5-10.0 and 5-42 degrees C; optimum growth was observed with 3-8 % (w/v) total salts and at pH 6.5-8.0 and 28-37 degrees C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2(T) to the genus Jeotgalicoccus. The predominant menaquinone of strain NY-2(T) was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C(15 : 0) (49.0 %) and anteiso-C(15 : 0) (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2(T) was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2(T) formed a coherent cluster with Jeotgalicoccus marinus JSM 076033(T), Jeotgalicoccus halotolerans YKJ-101(T) and Jeotgalicoccus psychrophilus YKJ-115(T). Phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2(T) (=CCTCC AB 208288(T) =JCM 15687(T)) should be classified as the type strain of a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus huakuii sp. nov. is proposed.