Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus

Abstract

A Gram-stain-positive, endospore-forming, motile, catalase- and oxidase-positive, aerobic, rod-shaped bacterium, designated strain JSM 081008(T), was isolated from non-saline forest soil in China. Strain JSM 081008(T) was able to grow with 0-20% (w/v) NaCl, at pH 6.0-10.5 and at 10-45 degrees C; optimum growth was observed with 2-5% (w/v) NaCl, at pH 7.0-8.0 and at 30-35 degrees C. The peptidoglycan type was A1alpha linked directly through L-Lys. The major cellular fatty acids (>10% of the total) were anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:0. The predominant respiratory quinone was menaquinone 7 and the genomic DNA G + C content of the strain was 42.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 081008(T) should be assigned to the genus Jeotgalibacillus and was related most closely to the type strains of Jeotgalibacillus alimentarius (sequence similarity 99.4%) and Jeotgalibacillus salarius (97.0%), followed by Jeotgalibacillus campisalis (95.4%) and Jeotgalibacillus marinus (95.2%). The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081008(T) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed. The type strain is JSM 081008(T) (=DSM 22174(T) = KCTC 13528(T)). An emended description of the genus Jeotgalibacillus is also presented.