Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively

Abstract

A Gram-variable, motile and rod-shaped bacterial strain, ASL-1(T), was isolated from a marine saltern located on the coast of the Yellow Sea, Korea. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ASL-1(T) clustered with Jeotgalibacillus alimentarius YKJ-13(T) and that this cluster joined the clade comprising the type strains of two Marinibacillus species. Strain ASL-1(T) exhibited 16S rRNA gene sequence similarity values of 97.3 % to J. alimentarius YKJ-13(T) and 96.5 % to the type strains of Marinibacillus marinus and Marinibacillus campisalis. The chemotaxonomic properties of strain ASL-1(T) were similar to those of one or two of the genera Jeotgalibacillus and Marinibacillus. The peptidoglycan type was A1alpha linked directly through L-lysine as the diamino acid. Strain ASL-1(T) contained MK-7 as the predominant menaquinone with the presence of a significant amount of MK-8. The predominant fatty acid was anteiso-C(15 : 0). The DNA G+C content was 42.9 mol%. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain ASL-1(T) could be differentiated from J. alimentarius and the two Marinibacillus species. On the basis of the data presented, strain ASL-1(T) represents a novel species within the genus Jeotgalibacillus, for which the name Jeotgalibacillus salarius sp. nov. is proposed. The type strain is ASL-1(T) (=KCTC 13257(T)=CCUG 56751(T)). It is also proposed that Marinibacillus marinus and Marinibacillus campisalis be reclassified as Jeotgalibacillus marinus comb. nov. (type strain 581(T)=DSM 1297(T)=ATCC 29841(T)=CCUG 28884(T)=CIP 103308(T)=LMG 6930(T)) and Jeotgalibacillus campisalis comb. nov. (type strain SF-57(T)=KCCM 41644(T)=JCM 11810(T)), respectively.