Jasmonic acid levels decline in advance of the transition to the adult phase in maize.

Abstract

Leaf‐derived signals drive the development of the shoot, eventually leading to flowering. In maize, transcripts of genes that facilitate jasmonic acid (JA) signaling are more abundant in juvenile compared to adult leaf primordia; exogenous application of JA both extends the juvenile phase and delays the decline in miR156 levels. To test the hypothesis that JA promotes juvenility, we measured JA and meJA levels using LC‐MS in successive stages of leaf one development and in later leaves at stages leading up to phase change in both normal maize and phase change mutants. We concurrently measured gibberellic acid (GA), required for the timely transition to the adult phase. Jasmonic acid levels increased from germination through leaf one differentiation, declining in later formed leaves as the shoot approached phase change. In contrast, levels of GA were low in leaf one after germination and increased as the shoot matured to the adult phase. Multiple doses of exogenous JA resulted in the production of as many as three additional juvenile leaves. We analyzed two transcript expression datasets to investigate when gene regulation by miR156 begins in the context of spatiotemporal patterns of JA and GA signaling. Quantifying these hormones in phase change mutants provided insight into how these two hormones control phase‐specific patterns of differentiation. We conclude that the hormone JA is a leaf‐provisioned signal that influences the duration, and possibly the initiation, of the juvenile phase of maize by controlling patterns of differentiation in successive leaf primordia.