Abstract
The interplay of plant hormones and glucose (Glu) in regulating glucosinolate accumulation in Arabidopsis thaliana was investigated in this study. Glucose-induced glucosinolate biosynthesis was enhanced significantly by the addition of jasmonic acid (JA), whereas the synergistic effect of salicylic acid (SA) and Glu was less obvious. The enhanced glucosinolate accumulation is associated with elevated expression of genes in glucosinolate biosynthetic pathway, as well as the transcription factors involved in their regulation, such as MYB28, MYB29, MYB34, and MYB122. The induction of indolic and aliphatic glucosinolates after treatment with JA and Glu in JA-insensitive mutants, coi1, jar1, and jin1, was compromised. Moreover, the effect of JA and Glu on glucosinolate contents was dramatically reduced in Glu-insensitive mutants, rgs1-2 and abi5-7. These results indicate a crosstalk between JA and Glu signalling in the regulation of glucosinolate biosynthesis. JA signalling, RGS1 (the putative membrane receptor of Glu signalling), and ABI5, are involved in the synergistic effect of JA and Glu on glucosinolate accumulation.
Highlights
Glucosinolates are a group of secondary metabolites containing nitrogen and sulphur elements
These results indicate a crosstalk between jasmonic acid (JA) and Glu signalling in the regulation of glucosinolate biosynthesis
Little is known about the synergistic effect of JA and Glu in inducing glucosinolate biosynthesis, the regulation of JA or methyl JA on indolic glucosinolate metabolism in Brassica species has been reported (Bodnaryk, 1994)
Summary
Glucosinolates are a group of secondary metabolites containing nitrogen and sulphur elements. They exist mainly in plants of the order Brassicales (Bones and Rossiter, 1996). Glucosinolates and their hydrolysis metabolites are involved in diverse functions such as flavour (Baik et al, 2003), chemoprotection (Bradburne and Mithen, 2000; Mithen et al, 2000; Clay et al, 2009), antioxidant activity (Keck and Finley, 2004), and resistance to biotic and abiotic stresses (Brader et al, 2006; Hopkins et al, 2009; Hirayama and Shinozaki, 2010). In the biosynthesis of glucosinolate core structure, substrates tryptophan and methionine are first metabolized by CYP79B2/CYP79B3 and CYP79F1/CYP79F2 (Chen et al, 2003) to the corresponding aldoximes, respectively.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
