Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in 'Zaosu' Pear (Pyrusbretschneideri Rehd.) Fruit.

Ting Wu,Ling-Fei Xu,Cheng-Quan Yang,Rui Zhai,Han-Ting Liu,Jun-Xing Song,Guang-Ping Zhao,Feng-Wang Ma,Xiao-Li Wang,Zhi-Gang Wang

doi:10.3390/biom10020278

Ting Wu, Ling-Fei Xu + Show 8 more

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https://doi.org/10.3390/biom10020278

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Journal: Biomolecules	Publication Date: Feb 11, 2020
Citations: 23	License type: CC BY 4.0

Affiliation: North West Agriculture and Forestry University

Abstract

Anthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, PbERF22 was identified from the lanolin-induced red fruit of ‘Zaosu’ pear (Pyrus bretschneideri Rehd.) using a comparative transcriptome analysis. Its expression level was up- and down-regulated by methyl jasmonate and 1-methylcyclopropene plus lanolin treatments, respectively, which indicated that PbERF22 responded to the jasmonate- and ethylene-signaling pathways. In addition, transiently overexpressed PbERF22 induced anthocyanin biosynthesis in ‘Zaosu’ fruit, and a quantitative PCR analysis further confirmed that PbERF22 facilitated the expression of anthocyanin biosynthetic structural and regulatory genes. Moreover, a dual luciferase assay showed that PbERF22 enhanced the activation effects of PbMYB10 and PbMYB10b on the PbUFGT promoter. Therefore, PbERF22 responses to jasmonate and ethylene signals and regulates anthocyanin biosynthesis. This provides a new perspective on the correlation between jasmonate–ethylene crosstalk and anthocyanin biosynthesis.

Highlights

Anthocyanins, an important class of plant secondary metabolites, play important roles in plant growth and development and in resistance to environmental stresses [1]
A series of enzymes encoded by structural genes (PAL, CHS, CHI, F3H, DFR, ANS, and UFGT) are involved in anthocyanin biosynthesis, and these structural genes are co-regulated by the MBW transcription complex composed of myeloblastosis (MYB), basic helix-loop-helix proteins, and WD40 proteins
Arabidopsis thaliana ZAT6 directly binds the promoters of MYB12 and MYB111 to activate the expression levels of genes involved in anthocyanin biosynthesis in plants treated with H2O2 [12]

Summary

Introduction

Anthocyanins, an important class of plant secondary metabolites, play important roles in plant growth and development and in resistance to environmental stresses [1]. Aoguan) is similar to MYB10, and it interacts with bHLH3 to promote anthocyanin biosynthesis in pear [8]. In Prunus, the overexpression of MYB10.1/bHLH3 and MYB10.3/bHLH3 increases the expression levels of NtCHS, NtDFR, and NtUFGT to promote anthocyanin synthesis [11]. In addition to the MYB-bHLH-WD40 (MBW) complex, other transcription factors can coordinately regulate the expression of genes involved in anthocyanin synthesis. Arabidopsis thaliana ZAT6 directly binds the promoters of MYB12 and MYB111 to activate the expression levels of genes involved in anthocyanin biosynthesis in plants treated with H2O2 [12]. MdWRKY40 interacts with MdMYB1 to promote wounding-induced anthocyanin biosynthesis in apple [13]. PpHY5 directly binds to the promoter regions of PpCHS, PpDFR, PpANS, and PpMYB10, thereby promoting anthocyanin accumulation [14]

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