Japanese encephalitis vaccine-facilitated dengue virus infection-enhancement antibody in adults.

Yuka Saito,Meng Ling Moi,Masayuki Saijo,Kuniaki Hosono,Nozomi Takeshita,Tomohiko Takasaki,Hajime Shiba,Ichiro Kurane,Chang-Kweng Lim

doi:10.1186/s12879-016-1873-8

Abstract

BackgroundDengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the genus Flavivirus, and infection with a virus within this genus induces antibodies that are cross-reactive to other flaviviruses. Particularly in DENV infection, antibodies to DENV possess two competing activities: neutralizing activity and infection-enhancing activity. These antibody activities are considered central in modulating clinical outcomes of DENV infection. Here, we determined the neutralizing and infection-enhancing activity of DENV cross-reactive antibodies in adults before and after JE vaccination.MethodsParticipants were 77 Japanese adults who had received a single dose of inactivated Vero cell-derived JE vaccine. A total of 154 serum samples were obtained either before or approximately a month after a single dose of JE vaccination. The antibody-dependent enhancement (ADE) activity to each of four DENV serotypes and the neutralizing activities to DENV and to JEV were determined in each of the serum samples by using baby hamster kidney (BHK) cells and FcγR-expressing BHK cells.ResultsA total of 18 post-JE immunization samples demonstrated cross-reactivity to DENV in an anti-DENV IgG ELISA. DENV neutralizing antibodies were not detected after JE vaccination in this study. However, undiluted post-JE vaccination serum samples from 26 participants demonstrated monotypic and heterotypic ADE activity to DENV. ADE activity was also observed in 1:10-diluted samples from 35 of the JE vaccine recipients (35/77, 45 %).ConclusionIn summary, JE vaccination induced DENV cross-reactive antibodies, and at sub-neutralizing levels, these DENV cross-reactive antibodies possess DENV infection-enhancement activity. The results also indicate that cross-reactivity to DENV is associated with high levels of JEV neutralizing antibodies and, the DENV cross-reactivity is further facilitated by JE vaccination.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-016-1873-8) contains supplementary material, which is available to authorized users.

Highlights

Dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the genus Flavivirus, and infection with a virus within this genus induces antibodies that are cross-reactive to other flaviviruses
After JE vaccination, anti-DENV IgG antibodies was detected in 20 % (1/5) samples without JEV neutralizing activity (PRNT50 < 1:10), and in 40 % (29/72) samples with JEV neutralizing activity (Fig. 1a)
The results indicate that cross-reactive activity to DENV increase with JE vaccination and these activities possess antibody-dependent enhancement (ADE) activity to DENV

Summary

Introduction

Dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the genus Flavivirus, and infection with a virus within this genus induces antibodies that are cross-reactive to other flaviviruses. JE occurs in East, South, and Southeast Asia Both DENV and JEV cause serious public health threats and are the leading causes of hospitalization and death in children in vast regions of Asia. These viruses belong to the genus Flavivirus, and infection with a virus within this genus induces antibodies that are cross-reactive to other flaviviruses [4,5,6]. This mechanism leads to high levels of infection and the production of progeny viruses in DENV target

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