Janthinobacterium tructae sp. nov., Isolated from Kidney of Rainbow Trout (Oncorhynchus mykiss).

Won Joon Jung,Sung Bin Lee,Jin Woo Jun,Hyoun Joong Kim,Jeong Woo Kang,Se Chang Park,Jun Kwon,Sib Sankar Giri,Sang Wha Kim,Sang Guen Kim,Woo Taek Oh

doi:10.3390/pathogens10020229

Abstract

This study presents a novel Janthinobacterium strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to Janthinobacterium svalbardensis JA-1T. However, biochemical analysis found differences in D-xylose adonitol, N-acetylglucosamine, arbutin, and cellobiose. As for genome-to-genome distance and average nucleotide identity values calculated between strain SNU WT3 and other related strains such as J. lividum EIF1, J. svalbardensis PAMC 27463, and J. agaricidamnosum BHSEK were all below the cutoff value between species. DNA-DNA hybridization between strain SNU WT3 and other close relatives indicated the results of J. lividum DSM 1522T (47.11%) and J. svalbardensis JA-1T (38.88%) individually. The major fatty acid compositions of strain SNU WT3 were cylco-C17:0 (41.45%), C16:0 (33.86%) and C12:0 (5.87%). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and diphosphatidylglycerol. The quinone system was composed mainly of ubiquinone Q-8. The genome of strain SNU WT3 consists of 6,314,370 bp with a G + C content of 62.35%. Here, we describe a novel species of the genus Janthinobacterium, and the name Janthinobacterium tructae has been proposed with SNU WT3T (=KCTC 72518 = JCM 33613) as the type strain.

Highlights

IntroductionMembers of the genus Janthinobacterium are Gram-negative, rod-shaped, psychrotolerant bacteria normally found in environments such as soil, water, and the Arctic glaciers [1,2]
Members of the genus Janthinobacterium are Gram-negative, rod-shaped, psychrotolerant bacteria normally found in environments such as soil, water, and the Arctic glaciers [1,2].Janthinobacterium lividum, one of the earliest discovered Janthinobacterium species, was reported in 1978, and the species is found frequently in our nearest environment, including green onions, salad mix, water, and soil [3,4]
Of the reported complete genome data, all Janthinobacterium spp. strains and some other related species were used for average nucleotide nucleotide identity identity (ANI) and genome-to-genome distance distance calculator calculator (GGDC) analysis of SNU WT3, and the results indicated that strain SNU WT3 is a novel species

Summary

Introduction

Members of the genus Janthinobacterium are Gram-negative, rod-shaped, psychrotolerant bacteria normally found in environments such as soil, water, and the Arctic glaciers [1,2]. Janthinobacterium lividum, one of the earliest discovered Janthinobacterium species, was reported in 1978, and the species is found frequently in our nearest environment, including green onions, salad mix, water, and soil [3,4]. Since the discovery of J. lividum, only a few more Janthinobacterium species have been reported. Janthinobacterium svalbardensis was identified in 2013, isolated from ice samples of the island. Janthinobacterium psychrotolerans S3-2T , isolated from a freshwater pond near Aarhus, Denmark, was described in 2017 [7]. Three more species were described in 2020 from tropical and subtropical rivers of China: Janthinobacterium violaceinigrum

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Conclusion