J028011 細胞間接着が軟骨組織形成に及ぼす影響

Abstract

We reported that we could treat the defect of patella by using fibroin which is from silkworm cocoon. And we also reported that we observed the rich cartilage matrixes and chondrocyte's aggregation in fibroin. So we focused on the chondrocyte's aggregation on fibroin and tried to develop the method of analyzing it quantitatively for evaluating the effect of cell-cell interaction on aggregated chondrocyte cultured on fibroin or other matrixes. First, we tried to define cell aggregation by comparing chondrocyte's aggregation on fibroin with random walk models. And second, we cultured chondrocytes on fibroin, fibronectin and collagen type I and on we evaluated the amount of GAG production and the number of chondrocytes cultured on each matrixes. As a result, at first we found that on fibroin the number of cells which are 10-20 |0.m away from other cells is especially high. And also, they keep cell-cell adhesion for more than an hour. And second, it is suggested that the chondrocyte on fibroin is easier to aggregate than that on fibronectin or collagen type I. What is more, the chondrocyte on fibroin has a higher ability to produce GAG than that on other matrixes. From these results it is suggested that the aggregation movement on fibroin is caused by cell-cell adhesion but also such cell-cell interaction is affected by cell-matrix interaction. In addition it is also suggested that the aggregation movement is related to the ability of chondrogenesis.