Abstract
This paper presents a measurement method for Young's modulus of cells based on directly squeezing cells. A microfluidic cell squeezing device is used for measurement of the Young's modulus of cell. This device is designed so as to control the magnitude of pressure to cells. This device is made of transparent materials which permit in-situ monitoring of cellular deformation behavior by an optical microscope. Mechanical stress is directly applied to the cell surface with the deflection of the PDMS film above the cell culture chamber. Cells were gradually deformed within an elastic deformation range when the mechanical stress was applied. After eliminating the applied pressure, these cells were returned to the initial shape. The osteoblastic cells (MC3T3-E1) were used as target cell to evaluate the Young's modulus. Young's modulus of the osteoblastic cell was determined based on the cell deformation and the applied pressure. The result of Young's modulus of osteoblastic cells obtained from directly squeezing experiment was similar to the published values.
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