Abstract
In this study, we have developed a microfluidic device to control chemical stimulation to a cell population at a single cell scale for cell-based assay. The device consists of a cell culture chamber and microfluidic probe (MFP) channels which are possible to control pinpoint chemical stimulation in the microspace. The device has been evaluated by a computer simulation using Finite Element Method (FEM) and a flow control experiment. A control of chemical stimulation between a single cell scale and a cell population scale in the chamber can be possible by regulating flow ratio and channel size of the microfluidic probe. By using the method, we succeeded staining of single cell in a microspace. We conclude that the device can be a powerful tool for biological applications such as the differentiation control of pluripotent stem cells and other cells signaling studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Proceedings of Mechanical Engineering Congress, Japan
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
