J0201-1-3 表在性膀胱がんに対する薬剤送達法の開発(ドラッグデリバリーシステムとその周辺技術の基礎と展開(1))

Abstract

Seventy percent of patients with bladder cancer present superficial tumors with high recurrence rate. In order to develop effective superficial bladder cancer gene therapy, effective gene delivery using nanobubbles (NBs) and ultrasound (US) is our ultimate goal. To achieve this goal, we propose localized gene delivery in bladder using NBs and low-high-intensity US (LHUS) while low-intensity US direct NBs proximate to targeted cells in the bladder and high-intensity US collapse NBs to increase cell membrane permeability resulting the entry of exogenouse molecules into proximate cells. In the present study, to demonstrate the effectiveness of NBs and LHUS to localize the gene delivery to a designated bladder wall, we have assessed: i) in vivo local delivery and fragmentation of NBs with high frequency US imaging system; ii) localized molecular delivery of fluorescent molecules and quantification of the transfection efficacy; and iii) transfection of plasmid DNA and quantification of the transfection efficacy.