Abstract
Objectives:The purpose of this study was to determine the species composition of Eimeria circulating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay.Materials and Methods:Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromate from litter slurry collected from 13 commercially active broiler farms in the research region. Genomic DNA was isolated from sporulated oocysts and used to amplify the Eimeria species-specific ITS1 gene by PCR amplification. Electrophoresis of 1.5% agarose gel was used to visualize the amplified PCR products.Results:In the study samples from Mymensingh district, Bangladesh, the presence of Eimeria brunetti, Eimeria acervulina, Eimeria necatrix, Eimeria mitis, and Eimeria tenella was identified.Conclusions:The findings of this study may shed light on the zonal approach to chicken coccidiosis control. Additionally, it suggests that ITS1-based PCR might be used in the field to accurately identify Eimeria species.
Highlights
The composition and dynamics of Eimeria spp., the causative agent of chicken coccidiosis, are critical factors in determining the severity of the disease [1]
E. brunetti, E. acervulina, E. necatrix, E. mitis, and E. tenella of seven widely available species were identified in litter samples from broiler farms in Mymensingh, Bangladesh, using the Internal Transcribed Spacer 1 (ITS1)-polymerase chain reaction (PCR) method (Fig. 1)
The abundance of all seven chicken Eimeria species was reported based on oocyst morphology in Mymensingh district [10] and partial gene sequences described amplified region marker in the Chittagong district [11]
Summary
The composition and dynamics of Eimeria spp., the causative agent of chicken coccidiosis, are critical factors in determining the severity of the disease [1]. Coccidiosis in hens is one of the most prevalent and economically significant poultry diseases, causing an estimated $13.6 billion USD annual losses [2]. Selective Eimeria species infections can be catastrophic and destructive for chicken farms, depending on their prevalence, fecundity, and pathogenicity. The presence of highly pathogenic species, combined with their density, may predispose to coccidiosis epidemics and associated sickness. This infection has the potential to cause significant harm to the host’s gut, raising morbidity, mortality, and the risk of subsequent infections [3,4,5]. With an average frequency of 34.48% at the bird level [6], chicken coccidiosis appears to be a major constraint on Bangladesh’s poultry output
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Advanced Veterinary and Animal Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
