Abstract

Hackberry (Celtis L.) is subordinate to the Ulmaceae. The taxonomy of the species is difficult because of the high similarities of the leaf appearance shared by species. This study examined the systematics of Taiwanese Celtis based on the morphological characters of leaves and molecular evidence of nuclear ribosomal ITS region. In totally, 192 samples were sampled from 27 areas in Taiwan. Observation and selection of appropriate representations of leaf characters were made and 13 features were accordingly chosen based on morphological traits. An unweighting pair group method with arithmetic mean (UPGMA) dendrogram was constructed. In this study, specific primers were designed for the entire internal transcribed spacer region (ITS)and 5.8S ribosomal DNA(rDNA)gene were PCR amplified. A maximum parsimony tree was reconstructed based on ITS sequence. Five Celtis species, C. sinensis, C. formosana, C. nervosa, C. biondii and C. philippensis, were identified.

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