Abstract
Long-term consumption of sodium arsenite contaminated water can cause endemic arsenic disease. The proteome profile changes of liver fibrosis after exposure to arsenite containing water remain unclear. In this study, Sprague-Dawley (SD) male rats were treated with sodium arsenite (iAs3+), using a daily dose of 1.36 mg/kg body weight (medium dose group, M), 2.73 mg/kg body weight (high dose group, H) or deionized water (control group, C). Isobaric tags for relative and absolute quantitation (iTRAQ) were used to identify the different abundant proteins (DAPs) after arsenic-induced liver fibrosis. A total of 2987 high-quality proteins were detected (95% confident peptides ≥ 2), 608 of which were differentially expressed (fold change > 2 and p < 0.05) in M group and 475 in H group. Moreover, 431 DAPs were found in both M and H groups and used in subsequent bioinformatic analyses. Gene ontology (GO) analysis revealed 4,709 GO terms could be mapped, among which purine binding, actin filament binding and protein kinase binding were the most enriched terms for molecular function category. In addition, protein-protein interaction analysis showed six clusters of interaction networks. Our data provided new insights into the proteome changes after arsenic-induced liver fibrosis in model rats.
Highlights
In nature, trivalent arsenite [As (III)] is the most common oxidation states for soluble arsenic[1]
Our results provide new insights into the proteome profile changes of liver fibrosis induced by sodium arsenite stress
In the subgroups of Haematoxylin Eosin (H&E) staining, the results of control group indicated that the structural integrity of liver cells and none of edema, degeneration and necrosis observed in the cells surrounding central veins
Summary
Trivalent arsenite [As (III)] is the most common oxidation states for soluble arsenic[1]. Proteomics in comparison to genomics is an ideal tool to discover different abundant proteins, as they reflect the actual activity with respect to metabolic reactions and regulatory cascades and provide more direct information about microbial activity than functional genes and even their corresponding messenger RNAs13. Isobaric tags for relative and absolute quantification (iTRAQ) are employed widely, with a proven value in discovery-based proteomics[14,15]. This technology allows for simultaneous protein identification and (relative) quantification obtained at the MS/. ITRAQ has especial advantages over conventional proteomics techniques because this one identifies and quantifies many proteins from the specific biological environments using label peptides able to be identified by sensitive mass spectrometers. Our results provide new insights into the proteome profile changes of liver fibrosis induced by sodium arsenite stress
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