Abstract
BackgroundPreeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia.MethodsTo gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS.ResultsBy using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, had the strongest effect on cardiovascular function. The expression of G6PD was upregulated in thoracic aorta tissues, as confirmed by Western blotting. The expression of two other vascular function-related proteins, cysteine- and glycine-rich protein 2 (CSRP2) and tubulin alpha-4 A (TUBA4A), was upregulated, as demonstrated by mass spectrometry (MS).ConclusionsAlthough the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers.
Highlights
Preeclampsia, a major complication of pregnancy, is characterized by a high incidence of fetal and maternal mortality [1]
No significant changes were observed at 6 months (Additional file 1: Fig. S1), significant differences were observed in 1-year-old rats
We found no significant difference in the contractile response of mesenteric arteries to Phe between adult rats born to preeclamptic mothers and control rats (Fig. 1B)
Summary
Preeclampsia, a major complication of pregnancy, is characterized by a high incidence of fetal and maternal mortality [1]. Previous studies have defined the condition as de novo elevation of blood pressure (BP) to > 140/90 mmHg after the 20th week of gestation followed by proteinuria (> 0.3 g/24 h or ≥ + 1 on dipstick analysis) [2, 3]. Geelhoed et al [9] demonstrated that gestational hypertension and preeclampsia were correlated with elevated diastolic blood pressure (DBP) and systolic blood pressure (SBP) in 9-year-old offspring. Tenhola et al [10] found that the 12-yearold offspring of preeclamptic mothers exhibited significantly elevated DBP and SBP relative to corresponding. Kajantie et al [12] reported a significant association between preeclampsia and an increased risk of stroke and found that severe preeclampsia is linked to the incidence of hypertension in adult offspring aged 60–70 years. We described the protein expression profiles of umbilical artery tissues from patients with preeclampsia
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