ITRAQ-Based Quantitative Proteomic Analysis of the Arabidopsis Mutant opr3-1 in Response to Exogenous MeJA.

Jiayu Qi,Zhen Li,Xiaoyun Zhao

doi:10.3390/ijms21020571

Jiayu Qi, Zhen Li + Show 1 more

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https://doi.org/10.3390/ijms21020571

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Abstract

Jasmonates (JAs) regulate the defense of biotic and abiotic stresses, growth, development, and many other important biological processes in plants. The comprehensive proteomic profiling of plants under JAs treatment provides insights into the regulation mechanism of JAs. Isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis was performed on the Arabidopsis wild type (Ws) and JA synthesis deficiency mutant opr3-1. The effects of exogenous MeJA treatment on the proteome of opr3-1, which lacks endogenous JAs, were investigated. A total of 3683 proteins were identified and 126 proteins were differentially regulated between different genotypes and treatment groups. The functional classification of these differentially regulated proteins showed that they were involved in metabolic processes, responses to abiotic stress or biotic stress, the defense against pathogens and wounds, photosynthesis, protein synthesis, and developmental processes. Exogenous MeJA treatment induced the up-regulation of a large number of defense-related proteins and photosynthesis-related proteins, it also induced the down-regulation of many ribosomal proteins in opr3-1. These results were further verified by a quantitative real-time PCR (qRT-PCR) analysis of 15 selected genes. Our research provides the basis for further understanding the molecular mechanism of JAs’ regulation of plant defense, photosynthesis, protein synthesis, and development.

Highlights

Jasmonic acid (JA) is a plant hormone that plays an integral role in the regulation of plant growth and development as well as in plant defense against wounding, herbivory attack, and other biotic and abiotic stresses [1]
The resulting fatty acid hydroperoxide is released from the plasma membrane to peroxisome, which is dehydrated by allene oxide synthase (AOS) and cyclized by allene oxide cyclase (AOC) to form the cyclopentenone 12-oxo-phytodienoic acid (9S,13S-OPDA)
We investigated the effects of exogenous methyl jasmonate (MeJA) on Arabidopsis using the JA synthesis deficient mutant opr3-1

Summary

Introduction

Jasmonic acid (JA) is a plant hormone that plays an integral role in the regulation of plant growth and development as well as in plant defense against wounding, herbivory attack, and other biotic and abiotic stresses [1]. When encountering insect herbivory attack, plants immediately undergo a series of physiological responses and initiate a rapid biosynthesis of JAs to trigger the transduction of a stress signal, which results in the activation of the wound defense mechanism [18,19,20]. Most plants derive their jasmonates from octadecanoid (18-carbon) fatty acid, while some plants produce jasmonates from hexadecanoid (16-carbon) fatty acid [21]. MeJA has been widely used to study jasmonates signaling pathways and the mechanisms of plant defense

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