Abstract
BackgroundAs one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper.MethodsTo understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress.ResultIn the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.
Highlights
Pepper (Capsicum annuum L.) is one of the crops with the largest cultivated area in China, which greatly contributes to the vegetable supply and farmers’ income
After 48 h of heat stress, the contents of proline and soluble sugar were increased in two pepper genotypes, while their contents in the HT genotype were higher compared with the HS genotype (Figs. 1B, 1C)
We studied the physiological and proteomic changes of HT and HS seedlings to uncover the molecular mechanisms in heat tolerance
Summary
Pepper (Capsicum annuum L.) is one of the crops with the largest cultivated area in China, which greatly contributes to the vegetable supply and farmers’ income. ITRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings. To understand the heat stress response mechanism of pepper, an iTRAQbased quantitative proteomic analysis was employed to identify possible heatresponsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. We investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. According to bioinformatic analysis of heatresponsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180
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