Abstract
BackgroundCoats' disease is an uncommon form of retinal telangiectasis, and the identification of novel proteins that contribute to the development of Coats' disease is useful for improving treatment efficacy. Proteomic techniques have been used to study many eye diseases; however, few studies have used proteomics to study the development of Coats' disease.MethodsIsobaric tagging for relative and absolute protein quantification (iTRAQ) was employed to screen differentially expressed proteins (DEPs) in the aqueous humor (AH) between stage 3A patients (n = 8), stage 3B patients (n = 14), stage 4 patients (n = 2) and control patients (n = 20). Differentially co-expressed proteins (DCPs) were present in all three stages of Coats' disease and were considered disease-specific proteins. These proteins were further analyzed using Gene Ontology (GO) functional annotations.ResultsA total of 819 proteins were identified in the AH, 222 of which were significantly differentially expressed (fold change > 2 and P < 0.05) in the samples from at least one stage of Coats' disease. Of the DEPs, 46 were found among all three stages of Coats' disease and the controls; therefore, they were considered Coats' disease-specific proteins (DCPs). A GO classification analysis indicated that the DCPs were closely related to structural molecule activity, cell adhesion molecule binding and receptor binding. Western blotting confirmed the expression levels of haptoglobin and apolipoprotein C-I were significantly up-regulated in Coats’ disease.ConclusionsThe 46 Coats' disease-specific proteins may provide additional insights into the mechanism of Coats' disease and represent potential biomarkers for identifying individuals with Coats' disease.
Highlights
Coats' disease is a form of abnormal telangiectasia that is primarily characterized by aneurysms of retinal vessels and excessive production of yellowish intraretinal and subretinal exudates
Coats' disease is an uncommon form of retinal telangiectasis, and the identification of novel proteins that contribute to the development of Coats' disease is useful for improving treatment efficacy
A total of 819 proteins were identified in the aqueous humor (AH), 222 of which were significantly differentially expressed in the samples from at least one stage of Coats' disease
Summary
Coats' disease is a form of abnormal telangiectasia that is primarily characterized by aneurysms of retinal vessels and excessive production of yellowish intraretinal and subretinal exudates. Direct coagulation eventually increases the subretinal exudates, which promotes secondary retinal detachment [1] as well as complications related to exudative retinal detachment [5] This technique is not effective for cases with severe exudative changes [6]. The levels of nitric oxide in the AH of patients with Coats' disease are elevated, indicating proteins involve in nitric oxide metabolism may affect Coats’ disease development [19]. These proteins, which include antioxidant and immunoregulatory proteins, are essential for regulating homeostasis and may play a crucial role in the pathogenesis of Coats' disease [20]. Proteomic techniques have been used to study many eye diseases; few studies have used proteomics to study the development of Coats' disease
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
