Abstract
Thaxtomin A (TA) is a phytotoxin secreted by Streptomyces scabies that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation–reduction process, response to stress, plant–pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.
Highlights
The potato (Solanum tuberosum) is the third most important food crop in the world after wheat and rice, and it is widely grown on all continents except for Antarctica [1]
It has been known that thaxtomin A (TA) can trigger programmed cell death (PCD) in the host [23]
To confirm the phenotype in the potato, the Solanum tuberosum leaves were treated with different concentrations of TA
Summary
The potato (Solanum tuberosum) is the third most important food crop in the world after wheat and rice, and it is widely grown on all continents except for Antarctica [1]. The Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology has been used in proteomics studies due to its high efficiency and accuracy in identifying differentially expressed proteins (DEPs) [32,33,34]. This technology is used to analyze proteomics in response to different biotic or abiotic stresses, including heat stress [35], salt stress [36], drought tolerance [37], rice response to Magnaporthe oryzae [38], defense response in cotton triggered by Rhizoctonia solani [39], and Arabidopsis response to Verticillium dahliae [40].
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