Abstract
BackgroundGrey mould is an important plant disease worldwide, caused by Botrytis cinerea, resulting in serious economic loss. Wuyiencin, a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic, has been demonstrated effectiveness against B. cinerea.ResultsWuyiencin treatment inhibited growth and sporulation of B. cinerea, specifically altering hypha morphology and intracellular structures. These changes were accompanied by differential expression (fold change > 2.0) of 316 proteins identified by iTRAQ-labelling LC-MS/MS analysis (P < 0.05). Up-regulation of 14 proteins, including carbohydrate metabolism proteins and cell wall stabilization proteins, was validated by parallel reaction monitoring (PRM). Down-regulation of 13 proteins was validated by PRM, including regulators of energy metabolism, nucleotide/protein synthesis, and the biosynthesis of mediators of plant stress and decay.ConclusionOur results confirm the inhibitory biological effects of wuyiencin on B. cinereal and elaborate on the differentially expressed proteins and associated pathways implicated in the capacity of wuyiencin to debilitate the growth and pathogenicity of grey mould. This study provides validated candidates for further targeted exploration with the goal of optimizing wuyiencin as a safe, low-toxicity agent for biological control.
Highlights
Grey mould is an important plant disease worldwide, caused by Botrytis cinerea, resulting in serious economic loss
Effects of wuyiencin on the growth and physiological status of Botrytis cinerea The standard B. cinerea strain B05.10 was inoculated onto potato dextrose agar (PDA) medium containing wuyiencin at final concentrations of 0 ppm, 50 ppm, 100 ppm and 200 ppm, and incubated at 20 °C
The results showed that treatment with progressively higher concentrations of wuyiencin impeded the growth and development of B. cinerea, including a reduction in the presence of pigmented hyphae (Fig. 1a, b) and the number of spores detected (Fig. 1c)
Summary
Grey mould is an important plant disease worldwide, caused by Botrytis cinerea, resulting in serious economic loss. Grey mould is a fungus known scientifically as Botrytis cinerea. It is a common cause of disease among over 200 plant species, including fruit and vegetable plants, and flowers. Because B. cinerea is widely distributed through the air, it can spread quickly causing widespread pathogenesis and major economic losses in agriculture [1]. Popular Botryticides include fludioxonil, tebuconazole, iprodione, boscalid, and benzimidazole. These and other agricultural antimicrobial agents function by destabilizing the cell membrane and inhibiting protein and nucleic acid synthesis as well as cellular respiration; resistance to these antagonistic properties is not uncommon.
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