ITRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling

Sherinmol Thomas,Rameshwar Sharma,Abhilash Barpanda,Rakesh Kumar,Sanjeeva Srivastava,Yellamaraju Sreelakshmi,Kapil Sharma

doi:10.1038/s41598-021-87208-9

Abstract

In plants, during growth and development, photoreceptors monitor fluctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR-light (FR) insensitive mutant (fri) carries a recessive mutation in Phytochrome A and is characterized by the failure to de-etiolate in continuous FR. Here we used iTRAQ-based quantitative proteomics along with metabolomics to unravel the role of Phytochrome A in regulating central metabolism in tomato seedlings grown under FR. Our results indicate that Phytochrome A has a predominant role in FR-mediated establishment of the mature seedling proteome. Further, we observed temporal regulation in the expression of several of the late response proteins associated with central metabolism. The proteomics investigations identified a decreased abundance of enzymes involved in photosynthesis and carbon fixation in the mutant. Profound accumulation of storage proteins in the mutant ascertained the possible conversion of sugars into storage material instead of being used or the retention of an earlier profile associated with the mature embryo. The enhanced accumulation of organic sugars in the seedlings indicates the absence of photomorphogenesis in the mutant.

Highlights

Plant development is intimately bound to the external light environment
It was reported that loss of phytochrome impacts core metabolism and over-accumulation of a large number of primary metabolites have been observed in leaves of the Arabidopsis phyBD and phy ABDE mutants[8]
Plants have evolved an array of photoreceptors, which capture a wide range of light spectrum and alter a myriad of physiological processes upon reception of the light signal[9,10]

Summary

Introduction

Plant development is intimately bound to the external light environment. Light drives photosynthetic carbon fixation and activates a set of signal-transducing photoreceptors that regulate plant growth and development. Other Calvin cycle enzymes like phosphoglycerate kinase and Fructose 1.6-bisphosphate aldolase (Solyc02g062340.3.1) exhibited a decreased abundance when treated under FR light compared to the control seedlings (Fig. 2B and Table 3).

Results

Conclusion