Abstract
Porous solids are widely used in chromatographic separations based on size and shape. Some pores are as large as proteins (for instance, in dextran- or agarose-based gel filtration), whereas others are as small as water molecules (as in molecular sieves used to keep organic solvents dry). Paukstelis has assessed the permeation properties of a self-assembled three-dimensional lattice constructed from four assembly strands [each 24 nucleotides (nt) long] and an 11-nt spacer strand. The estimated diameter of the largest internal solvent channel in crystals of this DNA array was 9 nm, which corresponds to a globular protein of 300 kD, but the measured size cutoff for negatively charged proteins appeared to be only one-10th this size (ovalbumin, no; carbonic anhydrase, yes), perhaps as a result of electrostatic interactions. Confocal microscopy revealed that the interior of a crystal soaked in a mixture of green fluorescent protein and a much bigger red maltose-binding protein was green and not red. — GJC
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
