Abstract
Plant Biotechnology JournalVolume 20, Issue 10 p. 1845-1846 Issue InformationOpen Access Issue Information First published: 21 September 2022 https://doi.org/10.1111/pbi.13625AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Front cover image: The cover image is the engineering of red betanin biosynthesis in N. benthamiana leaves (upper panel), transgenic rice calli (middle panel) and seedings (lower panel) using a novel multigene stacking system TGSII-UNiE. In this issue, Zhao et al develops a simple and fl exible TGSII-UNiE system for high-effi ciency assembly of long DNAs and multiple genes, which uses unique nucleotide sequence-guided nicking endonuclease (UNiE)-mediated DNA assembly (UNiEDA) method combined with improved Cre/loxP recombination-mediated TransGene Stacking II (TGSII) system. Due to its effi ciency, convenience and low price, the TGSII-UNiE system shows signifi cant application potential for plant functional genomics, synthetic biology, metabolic engineering. Cover illustration refers to the article in this issue (Zhao et al., 1983–1995). Volume20, Issue10October 2022Pages 1845-1846 RelatedInformation
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