Abstract
Front cover The cellular expression and functions of the sigma-1 receptor (S1R) in the central nervous system (CNS) still remain elusive due to a lack of appropriate tools. For the first time since its discovery over forty years ago, the sigma-1 receptor was specifically detected in all central nervous system (CNS) cell types in vivo by a newly established immunohistochemical protocol. We took advantage of S1R knockout mice to identify a commercial antibody (Ab#61994) specifically recognizing S1Rs in immunoblots. We further established a simple immunohistochemical antigen retrieval method employing 1% SDS to reliably detect S1Rs with Ab#61994 in brain vibratome sections, demonstrating a wide expression of S1R in neurons and all glial cell types. We also generated a new S1R flox mouse for Cre-dependent conditional deletion of S1Rs for functional analysis in vivo. Image content Brain sections from wild-type (WT) and S1R knockout (KO) mice were pre-treated with 1% SDS and immunostained by various S1R antibodies separately. Scale bars = 200 μm. Read the full article ‘Specific detection and deletion of the sigma-1 receptor widely expressed in neurons and glial cells in vivo’ by Q. Liu, Q. Guo, L-P. Fang, H. Yao, A. Scheller, F. Kirchhoff, W. Huang, (J. Neurochem. 2023, vol.164 (6), pp. 764–785) on doi:10.1111/jnc.15693
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