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IUBMB LifeVolume 72, Issue 2 p. 186-186 ISSUE HIGHLIGHTSFree Access Issue Highlights First published: 14 January 2020 https://doi.org/10.1002/iub.2223AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat 266 Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli Danilo Correddu, José de Jesús Montaño López, S. Andreas Angermayr, Martin J. Middleditch, Leo S. Payne, Ivanhoe K. H. Leung The translation of the genetic code into protein products is characterized by the identification of triplets called codons that allow the incorporation of an amino acid. The heterologous expression of human genes in bacterial cells can encounter translational problems due to the presence of codons that are normally used with low frequency in bacteria. In Escherichia coli the codons AGG and AGA are the less frequent and are known to cause translational problems, particularly when they are present in tandem. Consecutive AGG-AGA can lead to a premature stop in a sequence dependent manner causing the production of a truncated protein. REFERENCES 1Danilo Correddu, José de Jesús Montaño López, S. Andreas Angermayr, Martin J. Middleditch, Leo S. Payne, Ivanhoe K. H. Leung. Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli. IUBMB Life. 2019, 72, 266– 274. Volume72, Issue2February 2020Pages 186-186 ReferencesRelatedInformation