Abstract
Front cover: Electron micrograph of a synapse between cerebellar granule cells in culture. Image taken at 80000 magnification. Scale 0.72pixel / nm. In cerebellar granule cells, and using FM1-43 dye to follow SV recycling, two main populations of boutons that differ in their membrane recycling efficiency are observed. We have used Brefeldin A (BFA) to inhibit AP3/AP1-mediated recycling and to test whether the contribution of this pathway could account for such heterogeneous responses. Combining imaging techniques (FM1-43 and vGluT1-pH) and ultrastructural analyses, we found that the AP3/AP1 pathway regulates membrane retrieval and vesicle reformation, albeit exerting a distinct weight on the different populations of boutons depending on their functional maturation. Read the full article ‘Brefeldin A sensitive mechanisms contribute to endocytotic membrane retrieval and vesicle recycling in cerebellar granule cells’ by A. Rampérez, J. Sánchez-Prieto and M. Torres (J. Neurochem. 2017, vol. 141 (5), pp. 662–675) on doi: 10.1111/jnc.14017
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
