Abstract
Cover legend: Cholera toxin is enriched in plasma membrane tubules formed by cytoplasmic dynein. COS-7 cells were ATP depleted prior to labeling with Alexa555-CTxB and imaged live at 37°C on a confocal microscope. ATP depletion inhibits endocytic scission, leading to the generation of microns long, nascent endocytic tubules that are continuous with the plasma membrane. These invaginations are microtubule-dependent and form through persistent microtubule motor activity of cytoplasmic dynein providing a pulling force on the cell surface. Using this assay, Day et al. define a novel role for microtubule motors in tubulating the plasma membrane during clathrin-independent endocytosis. See Day et al. Traffic 2015; 16(6):572–590. Read the full article on doi:10.1111/tra.12269
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