Issue Cover (January 2020)

Abstract

Journal of NeurochemistryVolume 152, Issue 1 Issue CoverFree Access Issue Cover (January 2020) First published: 22 December 2019 https://doi.org/10.1111/jnc.14727 Read the full preface: ‘High specificity of widely used phospho-tau antibodies validated using a quantitative wholecell based assay’ by D. Li, Y. K. Cho (J. Neurochem. 2020, vol. 152 (1), pp. 122–135) on doi: 10.1111/jnc.14830 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Front cover: Phospho-tau antibodies are the most common tool for detecting site-specific tau phosphorylation. However, there is no quantitative measure of specificity that guides users. We developed a robust flow cytometry assay that determines a specificity parameter termed ϕ(phi), which measures the fraction of non-specific signal in antibody binding. We applied the assay to measure the specificity of widely used phospho-tau antibodies, and show that phospho-tau antibodies AT8, AT180, PHF-6, TG-3, and PHF-1 have nearly perfect specificity. Image content: Representative confocal image showing highly specific recognition of tau phosphorylated at Thr231 (red) among total tau (green), imaged using EGFP-tau. Read the full preface ‘High specificity of widely used phospho-tau antibodies validated using a quantitative wholecell based assay’ by D. Li, Y. K. Cho (J. Neurochem. 2020, vol. 152 (1), pp. 122–135) on doi: 10.1111/jnc.14830 Volume152, Issue1January 2020 RelatedInformation