Abstract
ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.
Highlights
The Caricaceae family consists of 35 species distributed in six genera (Badillo 2000)
Four genotypes of V. monoica and of J. spinosa, collected in Brazil, two genotypes of V. goudotiana that were kindly supplied by USDA, and bulks of hermaphrodite and female C. papaya were investigated in the divergence study
The primers were 15-18 bases long and the annealing temperatures optimized for each one
Summary
The Caricaceae family consists of 35 species distributed in six genera (Badillo 2000). Papaya (Carica papaya L.) is an important fruit crop in tropical countries, especially in Brazil, Vasconcellea species are considered important, as a source of desirable traits in papaya improvement (Manshardt and Wenslaff 1989, Drew et al 1998). Several compatibility levels are observed within Vasconcellea. They are drastically reduced in crosses with C. papaya (Warmke et al 1954, Jimenez and Horovitz 1958, Horovitz and Jimenez 1967, Mekako and Nakasone 1975, Manshardt and Wenslaff 1989). For a better understanding of the genetic relationships in Caricaceae, several molecular studies have been performed, suggesting a considerable genetic distance between Carica and Vasconcellea (Jobin-Decor et al 1997, Aradhya et al 1999, Olson 2002, Van Droogenbroeck et al 2004, Kyndt et al 2006)
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