Abstract
Molecular fingerprints of four different species of Curcuma, viz., C. amada, C. caesia, C. longa and C. zedoaria, found in Tripura were developed using Inter Simple Sequence Repeats. Twenty ISSR primers generated 116 loci amplified in the range of 200 - 5000 bp with an average of 5.8 alleles and 1.6 effective alleles per locus. The percentage of polymorphic band was found to be 86.29 with an average of 5.15 per primer. Based on UPGMA algorithm these four species are placed in two different clusters that validate the classification based on external and internal morphological characters. The polymorphic ISSR markers generated from this study will be useful for understanding the genetic relationship of different species of the genus Curcuma.
Highlights
The genus Curcuma belonging to the family Zingiberaceae comprises ca. 80 species and shows the widespread distribution from tropical Asia to Australia and South pacific region [1]
Rhizomes of four species of Curcuma, viz., C. amada Roxb., C. longa L., C. zedoaria (Christm.) Roscoe and C. caesia Roxb. found in wild state were collected from different geographical locations of Tripura (Table 1) and grown in the experimental garden of Department of Botany, Tripura University for experimental purposes
Twenty ISSR primers that were used to characterize the genetic diversity among the species yielded 116 fragments with an average of 5.8 alleles and 1.6 effective alleles per locus (Table 3)
Summary
The genus Curcuma belonging to the family Zingiberaceae comprises ca. 80 species and shows the widespread distribution from tropical Asia to Australia and South pacific region [1]. The genus Curcuma belonging to the family Zingiberaceae comprises ca. 80 species and shows the widespread distribution from tropical Asia to Australia and South pacific region [1]. The highest diversity of Curcuma has been found in India and Thailand and about 40 species are indigenous to India [2]. Different species of Curcuma have immense medicinal value and have been extensively used in indigenous system of medicine [3]-[6]. It is well documented that the position of the spike, presence of coma bract and the color of bract in Curcuma are the major distinctive traits for delineation of species [7]. Variation in the position of the spikes and bract color has been noted in some species of Curcuma [8].
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