Abstract
In this study, 25 flower samples of Parrotia subaequalis individuals were collected in Dalonggou of Yixing, Jiangsu. ISSR markers were used to analyze the genetic diversity of the 25 individuals. Six primers were selected and used for PCR amplification. The result showed that 59 discernible DNA fragments were amplified, among them 47 are polymorphic loci and the percentage of polymorphic loci (PPB) is 79.7%. By analysis with POPGENE32, the effective number of alleles (Ne) is 1.4697, Nei's gene diversity (H) is 0.2792, Shannon's Information index (I) is 0.4195, the Ewens-Watterson Neutral value is between 0.02 to 1, and more than half of the neutral test values are above 0.5. Through cluster analysis with NTSYSpc-2.1, genetic similarity coefficients of 25 samples are between 0.25 and 1 with an average of 0.71. From the results of this study we can get a conclusion that the genetic differences among the wild populations of P. subaequalis in Dalonggou of Yixing, Jiangsu were large and rich in diversity. The aim of this study is to reveal the genetic diversity of wild population of P. subaequalis from molecular level and provide a theoretical basis for the protection of this rare and endangered plant.
Highlights
Parrotia subaequalis, belonging to the genus Parrotia of the Hamamelidaceae family, is one of the oldest angiosperms
A total of 59 ISSR amplified bands were obtained by PCR amplification of 25 samples of P. subaequalis in Dalonggou, with good polymorphism, high stability and clear bands in the early stage of the research group
ISSR genetic diversity analysis was performed on the population of P. subaequalis in Dalonggou, Yixing, Jiangsu
Summary
Parrotia subaequalis, belonging to the genus Parrotia of the Hamamelidaceae family, is one of the oldest angiosperms. It is the only "living fossil" species that has been rediscovered, is endemic to China. P. subaequalis has been extinct because the number of its existing populations is extremely small. In 1999, it was listed in the national first-level protected wild plants list[3,4] and listed by the International Union for Conservation of Nature (IUCN) as a Critically Endangered (CR) species[5]. In recent years, the research work on P. subaequalis is active and most of the research is focused on morphological anatomy[6,7], population structure[8,9], biological characteristics[10,11,12], breeding and siring[13,14,15]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
