Abstract
Olive cultivar identification is very important especially for fruit productivity and olive oil quality. Up to date, numerous techniques have been used for characterization of olive cultivars and detection of genetic variability. In the present study, 40 clones specific to Turkey (8 cultivars, each having 5 clones) were assessed for cultivar determination via inter simple sequence repeat (ISSR) marker systems using 10 ISSR primers. In total, 217 band profiles were obtained, 206 of which were polymorphic (94.9% polymorphism). The cultivars were segregated into two main clusters, each cluster being subdivided into two groups, while all the clones of a cultivar belong to the same main cluster. The only exception was the distribution of the clones of cultivar 'Tekir': 'Tekir 1', 'Tekir 2' and 'Tekir 3' on cluster III, while 'Tekir 4' and 'Tekir 5' were on cluster IV, therefore grouped into different main clusters. In the present study, ISSR analysis displayed a high level of genetic variability among Turkish olive cultivars, indicating a potential resource for the use of this germplasm in clonal selection programs.
Highlights
Olive (Olea europea L.), with more than 2,600 cultivars, is one of the oldest cultivated plants characteristic of the Mediterranean area, where it is the most important oil-producing crop (Bartolini et al 2005; Rugini and Lavee, 1992; Zohary and Hopf, 1994)
A number of inter simple sequence repeat (ISSR) markers have already been developed in olive and their primer sequences have been published (Hess et al, 2000; Gemas et al, 2004)
This study aims to determination of genetic diversity and relationship in fourty Turkish olive (Olea europaea L.) clones belonging to eight cultivars via inter simple sequence repeat (ISSR) marker systems using ten ISSR primers
Summary
Olive (Olea europea L.), with more than 2,600 cultivars, is one of the oldest cultivated plants characteristic of the Mediterranean area, where it is the most important oil-producing crop (Bartolini et al 2005; Rugini and Lavee, 1992; Zohary and Hopf, 1994). It is determined in two forms, former is wild Olive trees have a high level of heterozygosity and genetic polymorphism is common among cultivars, so that they are predominantly allogamus (Angiolillo et al, 1999; Diaz et al, 2006; Rallo et al, 2000). A number of ISSR markers have already been developed in olive and their primer sequences have been published (Hess et al, 2000; Gemas et al, 2004)
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