Abstract
Three hundred and sixty-nine cultures of Histoplasma capsulatum were isolated by means of soil-inoculated mice from four disjunct sites, three consisting of blackbird roosts and one a gull nesting area. Repeatable isozyme patterns of eight enzyme systems were obtained by starch-gel electrophoresis. Isozyme polymorphism was observed among the isolates from each soil sample in at least one of the enzyme systems. High levels of isozyme variation were found within and between the sample sites. Isolates from the three blackbird sites were phenotypically more variable but showed an overall greater similarity to each other than to those from the gull site. Isolates from tissues of individual mice produced from two to ten different isozyme phenotypes, with an average of 40.5% of the isolates differing from the predominant phenotype.
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