Isozyme similarity and genetic differences in morphology between hyprosola, a high yielding, high protein mutant of chickpea ( Cicer arietinum L.) and its parental cultivar

Abstract

Twenty accessions of chickpea ( Cicer arietinum L.) were assayed for variation in electrophoretic mobility of 17 enzymes on starch gels. Only four of the 27 loci detected were polymorphic, one for three alleles and three for two alleles. The related annual wild species, C. judaicum Boiss, and C. cuneatum Hochst. ex Rich., differed isozymically from C. arietinum at 26 of the 27 loci. The mutant Hyprosola is identical for these isozymes to the minor component of its parental cultivar Faridpur-1, which was found from the isozyme survey to be a cryptic mixture of two genotypes at the Aco-2 locus. This result is consistent with Hyprosola being an induced mutant in Faridpur-1, rather than a rare contaminant. Analyses of F 2 segregation data indicated recessive mutant alleles in Hyprosola for small seeds ( ssd), long, narrow leaflets ( lnl) and early flowering ( efl). Reduced plant height in Hyprosola apparently results from recessive mutations at two loci, rht-1 and rht-2, one of which is linked to ssd with 16 ± 6% recombination. Hyprosola contains no deleterious mutations affecting ovule fertility. No mutants affecting seed development, other than ssd, were found.