Abstract
The Immunoglobulin IgG1 and IgG2 isotype immune responses of domestic ruminants and mice to Cowdria. ruminantium live infection or by immunization with inactivated organisms were determined by the enzyme linked immunosorbent assay and Western blotting. Immunization of goats with inactivated elementary bodies (IEBs) led to a predominant IgG1 isotype response. This indicated that a Th2 response was induced. After challenge, the IgG isotype responses were mixed whereby both IgG1 and IgG2 antibodies were detected. Two goats that survived virulent challenge had a predominant IgG2 isotype response. In cattle live infection by natural challenge or experiment led to a predominant IgG1 isotype response. Immunization of cattle with IEBs however led to mixed IgG responses characterized by similar IgG1 and IgG2 ratios. In the mouse live infection led to a predominant IgG2 isotype response. This indicated the mouse developed a true Th1 type cell mediated immune response when inoculated with live organisms. Immunization with inactivated organisms on the other hand led to a dominant IgG1 response. It is evident from this work that the immune responses of ruminants and mice to C. ruminantium are different and that using mice as the experimental model for immune responses to Cowdria ruminantium. is not the appropriate. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 4 : 541-548)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.