Isotopic Turnover and Fractionation of δ15N and δ13C in Captive Pseudopleuronectes americanus (Walbaum)

Abstract

Stable isotope ratios of nitrogen (δ15N) and carbon (δ13C) are ubiquitous ecological tracers used to elucidate an organism’s diet and habitat. However, the application of stable isotope ratios to reconstruct a consumer’s ecology relies upon accurate rates for isotopic turnover at both a tissue and species-specific level. This study estimated isotope turnover rates and trophic discrimination factors in four different tissues (liver, digestive tissue, muscle, and skin) with variable metabolic activity in winter flounder Pseudopleuronectes americanus using a controlled diet-switch experiment. Differences in half-lives were noted among the tissues and between the experimental diets for both δ15N and δ13C. The experimental diets of krill and mysis had variability in nutritional composition, resulting in similar turnovers in δ15N but slower turnovers in δ13C for fish fed krill. Turnovers in both δ15N and δ13C were strongly influenced by metabolism, with the contribution reaching up to 98%, as fish exhibited minimal overall growth. The results of this study demonstrate the importance of considering differences in the catabolic activity of tissue maintenance for fish exhibiting minimal growth, as well as differences in metabolic assimilation of dietary sources that vary in their protein and lipid contents.