Abstract
Mouse interferon produced by C-243 cells induced with Newcastle disease virus was isotopically labeled by adding either [ 35S]methionine or a 14C-labeled amino acid mixture to the culture medium. A method combining butyric acid and theophylline treatment and resulting in high interferon yields was used. Following purification by two-step affinity chromatography on poly(U) and antibody columns, the resulting material was analyzed on SDS-PAGE. The migration pattern of radioactivity and interferon coincided well and autoradiography revealed three major bands at migration distances corresponding, respectively, to 35, 28, and 22 K. Interferon represented 3.8% of all [ 35S]methionine-labeled proteins and 2.6% of all 14C-amino acid-labeled proteins released into the medium.
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