Abstract
A method for isotopic determination of silicon by mass spectrometry in plants and soils labeled with 30Si is reported. The development of this method is for use with studies involving the physiological process of absorption, transport, and redistribution of Si in the soil-plant system by use of the stable isotope 30Si as a tracer. The procedure leads to SiF4 formation, and the isotopic determination of Si was based on the measurements of the 28SiF3 +, 29SiF3 +, and 30SiF3 + signals. Relative standard deviation of 30Si abundance measurements (n = 6) were lower than 0.1%, and the detection limit was 0.5 mg Si (dry mass).
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