Isotopic (D/H) management of chemical and biological processes properties: applications in pharmaceutical engineering

Abstract

The strategy of sustainable industrial development in accordance with “green chemistry” stimulates researchers to search for a “lever” that could provide control over the direction and speed of chemical and biological processes. Water, depleted in deuterium content, is a recognized safe (in contrast to D2O) dissolution medium according to the results of numerous clinical observations. Due to the lower strength of carbon-protium (C-H) chemical bonds compared to carbon-deuterium (C - D), the properties of deuterated compounds, such as the rate of reactions involving them, change. The aim of the work was to study the effect of varying the 21H content on controlling the chemical and biological properties of processes. The highest values of kinetic deuterium isotope effects (KEI), which demonstrated greater sensitivity to the difference in the nucleolar mass D/H in the dissolution medium, correspond to slightly soluble lipophilic molecules. We also found the dependence of the S.ambigua cell biosensor lifetime on the deuterium concentration in the dissolution medium according to the laws of Arrhenius kinetics. Thus, the developed approach based on the laser diffraction technique in controlling the rate of chemical and biological processes can be successfully applied at all stages of the production cycle in the chemical and pharmaceutical industry.