Isotopic Composition (δ15N and δ18O) of Urban Forests in Different Climate Types Indicates the Potential Influences of Traffic Exhaust and Relative Humidity

Abstract

Previous studies have shown that climatic and anthropogenic factors influence the variations in the stable isotope signature of tree materials in an urban ecosystem. However, the responses of urban trees to various anthropogenic and precipitation interventions are unclear. To fill the research gap, the isotopic composition (δ15N and δ18O) and total nitrogen content of the soil-plant system of urban forests were measured, focusing on typical urban tree species (Populus tomentosa and Ficus virens). The research was done under various land uses in China’s Beijing and Shenzhen megacities (with different humidity conditions). The linear mixed-effect model revealed that the δ15N values in the leaves of roadside Ficus virens were significantly higher than those in urban park sites, away from the road (δ15N difference = 7.2‰). In contrast, Populus tomentosa leaves exhibited a non-significant difference (δ15N difference = −1.8‰). Further regression analysis revealed that the variations in δ15N in tree leaves could be attributed to the proximity to highways, suggesting the influence of vehicle exhaust on δ15N values in roadside trees. The linear mixed-effect model also revealed δ18O values significantly higher in Populus tomentosa leaves than in Ficus virens leaves. To assess the influence of humidity conditions on δ18O values in the leaves, the ratio of the atmospheric and intercellular vapor pressures (ea/ei) of tree canopies was estimated. The result revealed that the ea/ei of Ficus virens was significantly higher than for Populus tomentosa, ascribed to the variation in the humidity conditions of the two megacities. These results highlight that urban trees can potentially serve as bioindicators for atmospheric pollution and humidity. Our findings highlight the ecophysiological responses of urban forests related to N and O as they vary according to traffic exhaust and relative humidity. Consequently, they are potentially valuable indicators of urban atmospheric contamination, forming a nature-based solution for citizen welfare improvement.