Abstract

The determination of the natural variations of stable isotopic ratios of different substances in the biosphere can be considered as a complementary technique to tracer studies with enriched stable or radioactive isotopes. This is particularly true when we study the behaviour of a substance in its natural environment, where the use of tracers must be limited. In the water cycle in an ecosystem, plants play an important role due to their large surface area and water turnover of plants can be studied by hydrogen and oxygen isotope ratios. The biosynthesis of plants constituents can be characterized, in some favorable cases, by particular carbon or nitrogen isotope ratios. These phencmenons can be used successfully in a wide range of disciplines : plant physiology, human or animal metabolic studies, detection of adulteration of foods, origin of pollution.

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