Abstract
The use of oxygen isotopes revealed that when 2,6-dichlorophenolindophenol and ascorbic acid were added to a system containing illuminated Phytolacca chloroplasts functioning in a Mehler reaction, the rate of oxygen evolution was decreased to 9 10 while the rate of oxygen consumption was increased to 2 3 4 times the original rates. After complete oxidation of the added ascorbate, the rate of oxygen evolution increased to 1 1 2 times the original rate. These results support the assumption that ascorbate can be oxidized via the (OH) system produced by the photolysis of water.
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