Isotope analysis reveals proportional change and site‐selection variation of river‐ and lake‐produced eggs of a landlocked migratory fish

Abstract

Changes in the proportions of river- and lake-produced eggs of a landlocked amphidromous fish, ayu (Plecoglossus altivelis altivelis) in the Lake Biwa water system, Japan, were monitored by stable isotope analysis, based on different δ15 N and δ13 C values of prey organisms between the lake and its tributaries. During the 3 month reproduction season, the δ15 N values of spawned eggs decreased with time. This result implies that there was a shift from lake-produced eggs to river-produced eggs within a reproductive season, based on the observation that adult fish in the lake had previously been shown to have eggs with distinctly higher δ15 N values in their ovaries than those in the tributaries. This explanation was also supported by the change in δ13 C values of the spawned eggs. Furthermore, eggs with lower δ15 N and higher δ13 C values tended to be spawned at less variable depths, suggesting that females spawning river-produced eggs selected the spawning sites from a narrower range. We conclude that stable isotope ratios of spawned eggs can be indicators of the relative contributions of different food chains and can enable comparisons of reproductive characteristics between types of egg.