Isothiocyanato‐substituted hydrophilic polymer beads for immobilization of enzymes

Abstract

AbstractStyrene–glycidylmethacrylate–divinylbenzene resin beads could be converted to highly hydrophilic resin beads by acid treatment. These resin beads could be modified with isothiocyanato (NCS) groups quite easily using a Friedel–Crafts reaction and a subsequent nucleophilic reaction with KSCN. The resin beads substituted with NCS groups were useful as supports to immobilize enzymes, because they were not only hydrophilic but also bind proteins covalently. The enzymes immobilized onto the resin beads showed sufficiently high catalytic activities for a long period.